Current & past lab members – Proteomics Center // Erasmus MC

Dr. Jeroen Demmers

Director Proteomics Core Facility | Associate Professor

EUR Fellow, Erasmus University Medical Center

Postdoc, The Rockefeller University, New York (Chait lab)

PhD, Utrecht University (Heck & Killian labs)

MSc (hons) Chemistry, Utrecht University

More about Jeroen & contact information...

Research interests

mass spectrometry based proteomics
quantitation in proteomics (SILAC, TMT, etc.)
targeted proteomics
protein complex and protein-protein interaction networks
characterization of posttranslational protein modifications
the ubiquitin-proteasome system
transcription and gene regulation in health and disease
chromatin biology

Selected publications

Sijm A, Atlasi Y, van der Knaap JA, Wolf van der Meer J, Chalkley GE, Bezstarosti K, Dekkers DHW, Doff WAS, Ozgur Z, van IJcken WFJ, Demmers JAA, Verrijzer CP (2022) USP7 regulates the ncPRC1 Polycomb axis to stimulate genomic H2AK119ub1 deposition uncoupled from H3K27me3. Sci Adv 8(44):eabq7598. (ubiquitinomics | targeted proteomics | USP7)
Sap KA, Bezstarosti K, Dekkers DHW, Voets O, Demmers JAA (2017) Quantitative Proteomics Reveals Extensive Changes in the Ubiquitinome after Perturbation of the Proteasome by Targeted dsRNA-Mediated Subunit Knockdown in Drosophila. J Proteome Res 16(8):2848-2862. (global ubiquitinome | proteasome malfunctioning | ubiquitin | SILAC)
van den Berg DL, Snoek T, Mullin NP, Yates A, Bezstarosti K, Demmers J, Chambers I, Poot RA (2010) An Oct4-centered protein interaction network in embryonic stem cells. Cell Stem Cell 6(4):369-81. (Oct4 | interaction network)
Sap KA, Bezstarosti K, Dekkers DH, van den Hout M, van Ijcken W, Rijkers E, Demmers JAA (2015) Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster. Proteomics 15(4):725-38.
Karel Bezstarosti*, Alireza Ghamari*, Frank G. Grosveld, Jeroen A. A. Demmers (2010) Differential proteomics based on ¹⁸O labeling to determine the Cyclin Dependent Kinase 9 interactome. J Proteome Res 9(9):4464-75. (quantitation | ¹⁸O labeling | protein complex elucidation)
Raj VS, Mou H, Smits SL, Dekkers DH, Müller MA, Dijkman R, Muth D, Demmers JA, Zaki A, Fouchier RA, Thiel V, Drosten C, Rottier PJ, Osterhaus AD, Bosch BJ, Haagmans BL (2013) Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC. Nature 495(7440):251-4.
Adone Mohd-Sarip, Anna Lagarou, Ulku Aslan, Jan van der Knaap, Cecile Doyen, Karel Bezstarosti, Yasmin Yassin, Hugh W Brock, Jeroen AA Demmers & C. Peter Verrijzer (2012) Transcription-independent function of the Polycomb group protein PSC in cell cycle control. Science 336(6082):744-7. (PSC | protein complex elucidation)
Schwertman, P., Lagarou, A., Dekkers, D. H. W., Raams, A., van der Hoek, A. C., Laffeber, C., Hoeijmakers, J. H. J., Demmers, J. A. A., Fousteri, M., Vermeulen, W. & Marteijn, J. A. (2012) UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Nature Genetics 44(5):598-602. (quantitation | SILAC | ubiquitinome)

Teaching

Faculty member BSc Nanobiology (TU Delft / Erasmus MC) – courses Chemistry I NB1102 & Chemistry NB1110 II (6 ECTS)
Faculty member MSc Nanobiology (TU Delft / Erasmus MC) – course Engineering Genetic Information NB4030 – proteomics (3 ECTS)
Instructor and organizer of the MolMed PhD course ‘Biomolecular Research Techniques: Proteomics’
Instructor and organizer of the MGC PhD course ‘Technology Facilities: Proteomics’
Faculty member of the MSc Molecular Medicine research school
Guest lecturer at the Rotterdam University of Applied Sciences

Contact

j.demmers [at] erasmusmc.nl

📞 +31 10 7038124

Google Scholar

My NCBI PubMed bibliography

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Frontiers Loop

Researchgate

Dr. Nadine Prust

Proteomics scientist

PhD, Utrecht University (Heck Lab)

MSc, Ruhr University Bochum

BSc, Ruhr University Bochum

More about Nadine & contact information...

Research interests

mass spectrometry proteomics
characterization of posttranslational modifications
phosphoproteomics
Plasma / serum proteomics
Tissue proteomics

Selected publications

N Prust, PC van Breugel, S Lemeer (2022) Widespread Arginine Phosphorylation in Staphylococcus aureus. Mol Cell Proteomics 21(5): 100232- .
N Prust, S van der Laarse, HWP van den Toorn, NM van Sorge, S Lemeer, (2021) In-Depth Characterization of the Staphylococcus aureus Phosphoproteome Reveals New Targets of Stk1. Mol Cell Proteomics 20: 100034- .
CM Potel, MH Lin, N Prust, HWP van den Toorn, AJR Heck, S Lemeer (2019) Gaining Confidence in the Elusive Histidine Phosphoproteome. Anal Chem 91: 5542−5547.

Teaching

n.prust [at] erasmusmc.nl

📞 +31 10 7038124

Google Scholar

My NCBI PubMed bibliography

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Ing. Karel Bezstarosti

Proteomics scientist

Postgraduate internship, Heck lab Utrecht University

BSc, University of Applied Sciences, Rotterdam

More about Karel & contact information...

Research interests

Selected publications

Teaching

Instructor of the MolMed PhD course ‘Biomolecular Research Techniques: Proteomics’
Instructor of the MGC PhD course ‘Technology Facilities: Proteomics’

k.bezstarosti [at] erasmusmc.nl

📞 +31 10 7038124

Google Scholar

PubMed record

ORCiD, ResearcherID, Frontiers Loop

Ing. Dick Dekkers