Dr. Jeroen Demmers
Director Proteomics Core Facility | Associate Professor
EUR Fellow, Erasmus University Medical Center
Postdoc, The Rockefeller University, New York (Chait lab)
PhD, Utrecht University (Heck & Killian labs)
MSc (hons) Chemistry, Utrecht University
Research interests
- mass spectrometry based proteomics
- quantitation in proteomics (SILAC, TMT, etc.)
- targeted proteomics
- protein complex and protein-protein interaction networks
- characterization of posttranslational protein modifications
- the ubiquitin-proteasome system
- transcription and gene regulation in health and disease
- chromatin biology
Selected publications
- Sijm A, Atlasi Y
, van der Knaap JA, Wolf van der Meer J, Chalkley GE, Bezstarosti K, Dekkers DHW, Doff WAS, Ozgur Z, van IJcken WFJ, Demmers JAA, Verrijzer CP (2022) USP7 regulates the ncPRC1 Polycomb axis to stimulate genomic H2AK119ub1 deposition uncoupled from H3K27me3. Sci Adv 8(44):eabq7598. (ubiquitinomics | targeted proteomics | USP7)
- Sap KA, Bezstarosti K, Dekkers DHW, Voets O, Demmers JAA (2017) Quantitative Proteomics Reveals Extensive Changes in the Ubiquitinome after Perturbation of the Proteasome by Targeted dsRNA-Mediated Subunit Knockdown in Drosophila. J Proteome Res 16(8):2848-2862. (global ubiquitinome | proteasome malfunctioning | ubiquitin | SILAC)
- van den Berg DL, Snoek T, Mullin NP, Yates A, Bezstarosti K, Demmers J, Chambers I, Poot RA (2010) An Oct4-centered protein interaction network in embryonic stem cells. Cell Stem Cell 6(4):369-81. (Oct4 | interaction network)
- Sap KA, Bezstarosti K, Dekkers DH, van den Hout M, van Ijcken W, Rijkers E, Demmers JAA (2015) Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster. Proteomics 15(4):725-38.
- Karel Bezstarosti*, Alireza Ghamari*, Frank G. Grosveld, Jeroen A. A. Demmers (2010) Differential proteomics based on 18O labeling to determine the Cyclin Dependent Kinase 9 interactome. J Proteome Res 9(9):4464-75. (quantitation | 18O labeling | protein complex elucidation)
- Raj VS, Mou H, Smits SL, Dekkers DH, Müller MA, Dijkman R, Muth D, Demmers JA, Zaki A, Fouchier RA, Thiel V, Drosten C, Rottier PJ, Osterhaus AD, Bosch BJ, Haagmans BL (2013) Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC. Nature 495(7440):251-4.
- Adone Mohd-Sarip, Anna Lagarou, Ulku Aslan, Jan van der Knaap, Cecile Doyen, Karel Bezstarosti, Yasmin Yassin, Hugh W Brock, Jeroen AA Demmers & C. Peter Verrijzer (2012) Transcription-independent function of the Polycomb group protein PSC in cell cycle control. Science 336(6082):744-7. (PSC | protein complex elucidation)
- Schwertman, P., Lagarou, A., Dekkers, D. H. W., Raams, A., van der Hoek, A. C., Laffeber, C., Hoeijmakers, J. H. J., Demmers, J. A. A., Fousteri, M., Vermeulen, W. & Marteijn, J. A. (2012) UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Nature Genetics 44(5):598-602. (quantitation | SILAC | ubiquitinome)
Teaching
- Faculty member BSc Nanobiology (TU Delft / Erasmus MC) – courses Chemistry I NB1102 & Chemistry NB1110 II (6 ECTS)
- Faculty member MSc Nanobiology (TU Delft / Erasmus MC) – course Engineering Genetic Information NB4030 – proteomics (3 ECTS)
- Instructor and organizer of the MolMed PhD course ‘Biomolecular Research Techniques: Proteomics’
- Instructor and organizer of the MGC PhD course ‘Technology Facilities: Proteomics’
- Faculty member of the MSc Molecular Medicine research school
- Guest lecturer at the Rotterdam University of Applied Sciences
Contact
📞 +31 10 7038124