Consultation on experimental design – arrange an intake meeting with Jeroen Demmers
- Extraction of proteins from tissue, cell lysates, body fluids, IP-ed proteins, …
- Protein digestion (various proteases), also for top-down and middle-down protein analysis
Mass spectrometry (see Equipment for details)
- Orbitrap (Lumos Tribid, Fusion Tribid, Q Exactive Plus, XL ETD)
- Q-ToF
all in nanoflow LC configuration
- Protein identification
- Characterization of post-translational modifications
- Quantitative proteomics: LFQ, SILAC, TMT, 18O
- Targeted proteomics: MRM, PRM
Data analysis and bioinformatics solutions
- Software packages used: Proteome Discoverer (Thermo), Mascot (MatrixScience), MaxQuant/Perseus (Cox/Mann lab), R, customized software
Consultation on data interpretation
