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Erasmus MC theme* Biomedische WetenschappenDaniel den HoedDiagnostiek en AdviesDijkzigtGezondheidswetenschappenHersenen en ZintuigenSophiaSpoed, peri-operatief en intensiefThoraxRaad van BestuurServicebedrijfOther
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Proteins have been alkylated: NoYes
Proteins are: Separated on a gelIn solution or bound to beadsStill in (frozen) cell pelletsOther If other, please specify:
The gel is stained with: ---Coomassie/CBBSilverSypro rubyOther
Proteins have been digested already: NoYes If yes, the protease used was: ---TrypsinArg-CArg-NChymotrypsinLys-CCNBrV8Other If other, please specify:
What needs to be analyzed? Specific bands on gelComplete gel laneOn-bead digestComplete proteome (WCE)Modified proteomeSpecific protein targets only (e.g. PRM)Other If other, please specify:
Total number of samples*:
Name and number all your samples here*:
Uniprot ID of your co-IP bait protein:
Name of your co-IP bait protein, including all synonyms known to you:
Details about the antibody:
Cell line used:
Species*: HumanMouseRatDrosophilaOther
If other, please specify:
Type of quantitation requested*: NoneLabel free (LFQ)DIA LFQTargeted / PRMSILAC (K4/R6)SILAC (K6/R10)SILAC (K8/R10)Triple SILACTMT6TMT10TMTPro(16)Other
Analysis of post-translational modifications (always discuss with Proteomics Center staff first!):
Describe the goal of your experiment and the experimental protocols used to generate your samples in detail. Also, you can post any questions or comments here*:
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